구강편평세포암종에서 미슬토 추출물의 Apoptosis 유도 효과에 대한 실험적 연구

허균행; 이재훈; 김철환

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구강편평세포암종에서 미슬토 추출물의 Apoptosis 유도 효과에 대한 실험적 연구
An experimental study on mistletoe extract-induced apoptosis in oral squamous cell carcinoma

대한구강악안면외과학회지 2005년 31권 1호 p.13 ~ 23

허균행, 이재훈, 김철환,

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허균행 ( Heo Gyun-Haeng ) - 단국대학교 치과대학 구강악안면외과학교실
이재훈 ( Lee Jae-Hoon ) - 단국대학교 치과대학 구강악안면외과학교실
김철환 ( Kim Cheol-Whan ) - 단국대학교 치과대학 구강악안면외과학교실

KMID : 0355620050310010013

Abstract

This study was performed to investigate mistletoe extract-induced apoptosis in oral squamous cell carcinoma. In vivo study, HN22 cells were xenografted in nude mice. After tumor was experimentally induced, mistletoe extract was directly injected on the tumor mass. The specimens were evaluated using light and transmission electron microscopes. In vitro study, HN22 cells were cultured and exposed to mistletoe extract. The cells were evaluated using transmissin electron microscope. To evaluate apoptotic cells, flow cytometric analysis was done. The results were obtained as follows: 1. Light microscopic view of tumor mass showed necrosis at 2-4 weeks. 2. Transmission electron micrographs of tumor mass showed apoptosis and necrosis. 3. In TEM view of cell lines, necrosis and apoptosis were shown with mistletoe extract at 300?g/ml, apoptosis was shown with mistletoe extract at 100?g/ml. 4. In flow cytometric analysis, early and late apoptosis was shown when using caspase-3Ab and annexin-V, but no significant change was noted when using mebstain and Apo2.7 Ab. In this study, mistletoe extract induced necrosis and apoptosis in the tumor mass was induced by HN22 cells, early and late apoptosis in vitro study. Mistletoe extract was likely to induce cell death in oral squamous cell carcinoma through apoptosis.